This collection was originally compiled by Kent Christensen, Ph.D., J. Matthew Velkey, Ph.D., Lloyd M. Stoolman, M.D., Laura Hessler, and Diedra Mosley-Brower. Currently, it is curated by Michael Hortsch, Ph.D. If you have questions or comments regarding the University of Michigan virtual slide collection, please contact Dr. Hortsch at hortsch at umich.edu.
Virtual Slide List
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Resources
Access to the supplemental resources for this session is password-protected and restricted to University of Michigan students. If you are a University of Michigan student enrolled in a histology course at the University of Michigan, please click on the following link and use your Kerberos-password for access to download lecture handouts and the other resources.
Virtual Slide List for Histology Course
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185
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Taste Bud: Note the difference in cell shape and cell aggregation of the taste bud and the oral epithelium. The nerve endings are sensory nerves. Be able to recognize a taste bud as such, but you will not be required to know its detailed structure and recognize different cell types. | |
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187
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Mucous Acinus of a Lingual Salivary Gland: Note the accumulation of mucin-containing secretory vesicles next to the lumen of the secretory acinus depicted in this electron micrograph. |
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126
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Trachea & esophagus, cross section, H&E, 40X | |
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152
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Oral pharynx, H&E, 40X (stratified squamous non keratinized epithelium, lamina propria, elastic fibers, muscularis, mucous glands). | |
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152AF
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Oral pharynx, Masson trichrome stain, 40X (stratified squamous non keratinized epithelium, lamina propria, elastic fibers, muscularis, mucous glands). | |
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153
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Esophagus, H&E, 20X (stratified squamous non keratinized epithelium, lamina propria, muscularis mucosae, submucosa, submucosal mucous glands, muscularis externa [all smooth muscle], myenteric [or Auerbach's] plexus). | |
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155
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Esophagus and stomach, H&E, 40X (ESOPHAGUS [at right]: epithelium, lamina propria, muscularis mucosae, submucosa, muscularis externa [all smooth muscle], myenteric [or Auerbach's] plexus; CARDIAC STOMACH [next to esophagus]: pits, pyloric glands, muscularis mucosae; FUNDIC STOMACH [at left]: pits, glands, mucous cells, parietal cells [acid], chief cells [pepsinogen], muscularis mucosae, submucosa, muscularis). | |
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156
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Stomach, H&E, 20X ( pits, glands, mucous cells, parietal cells [acid], chief cells [pepsinogen], muscularis mucosae, submucosa, muscularis). | |
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156
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Stomach, H&E, 40X ( pits, glands, mucous cells, parietal cells [acid], chief cells [pepsinogen], muscularis mucosae, submucosa, muscularis). | |
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157
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Stomach, H&E, 20X ( pits, glands, mucous cells, parietal cells [acid], chief cells [pepsinogen], muscularis mucosae, submucosa, muscularis). | |
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157
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Stomach, H&E, 40X ( pits, glands, mucous cells, parietal cells [acid], chief cells [pepsinogen], muscularis mucosae, submucosa, muscularis). | |
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160
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Gastro-esophageal junction, PAS & Azure Blue stain, 40X | |
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162
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Pyloric stomach [at left], duodenum of small intestine [at right], H&E, 20X (pyloric pits and glands). | |
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162
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Pyloric stomach [at left], duodenum of small intestine [at right], H&E, 40X. | |
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190
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Stratified Squamous Non-keratinized Epithelium (Esophagus): Note the characteristic stratified squamous non-keratinized epithelial lining of the esophagus. | |
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197
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Gastric Pits and Glands (Fundus): Note that there is only one cell type, a mucous cell, in the surface epithelium of the stomach. The gastric pits lead into the gastric glands proper, where there are several cell types present. As always, the lamina propria consists of loose connective tissue. There is a rich capillary network in the lamina propria. | |
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198
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Mucous Surface Cell (Stomach Fundus): Mucous granules accumulate in the apical cytoplasm before they are released. | |
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203
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Chief Cell (Stomach Fundus): Review the structure of the chief cell. It is essentially that of an exocrine gland cell - as seen in the salivary glands and the pancreas. | |
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204
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Parietal Cell (Stomach Fundus): Note the abundant vesicles and tubules of the parietal cell, which are involved in HCl production. Note the numerous giant mitochondria in the parietal cell as opposed to the small and sparse mitochondria in the chief cells. Study the intracellular secretory canaliculi of the parietal cell. | |
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205
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Enteroendocrine Cell (Stomach): Note the accumulation of secretory vesicles in this enteroendocrine cell. They are usually found adjacent to the basal and lateral sides of the cell, not the apical aspect, which is facing the lumen of the gastrointestinal tract. You will not be asked to discriminate between different subtypes of enteroendocrine cells. |
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029
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Small intestine, H&E, 40X | |
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161
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Pylorus, duodenum, pancreas, H&E, 40X | |
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162
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Small intestine, duodenum [right side of section], H&E, 40X (villi very poorly preserved, glands [crypts of Lieberkühn], muscularis mucosae, submucosal glands). | |
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165
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Ileum, PAS stain, 40X | |
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168
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Small intestine, jejunum or ileum, H&E, 40X (plicae, villi, epithelium, goblet cells, lamina propria, intestinal glands [crypts of Lieberkühn], Paneth cells, muscularis mucosae, submucosa, muscularis externa, myenteric plexus), serosa). | |
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169
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Small intestine, jejunum or ileum, H&E, 20X (plicae, villi, epithelium, goblet cells, lamina propria, intestinal glands [crypts of Lieberkühn], Paneth cells, muscularis mucosae, submucosa, muscularis externa, myenteric plexus), serosa). | |
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169
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Small intestine, jejunum or ileum, H&E, 40X (plicae, villi, epithelium, goblet cells, lamina propria, intestinal glands [crypts of Lieberkühn], Paneth cells, muscularis mucosae, submucosa, muscularis externa, myenteric plexus), serosa). | |
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170
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Small intestine, ileum, H&E, 20X (contains nice Paneth cells). | |
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170
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Small intestine, ileum, H&E, 40X. | |
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171
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Small intestine, H&E, blood vessels injected with dye, 20X (observe small blood vessel distribution in villi and elsewhere). | |
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175
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Appendix, H&E, 40X (mucosa as in colon, prominent lymphoid nodules in submucosa). | |
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176
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Large intestine (colon), H&E, 40X (straight glands [crypts of Lieberkühn], no villi, simple columnar epithelium, goblet cells, lamina propria, muscularis mucosae, submucosa, muscularis externa, taenia coli). | |
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177
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Recto-anal junction, monkey, H&E, 40X | |
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177-2
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Rectoanal junction [anal canal at right], Masson trichrome, 20X (RECTUM: simple columnar epithelium in glands. ANAL CANAL: initially stratified columnar/cuboidal, then stratified squamous epithelium first non keratinized and then keratinized [thin skin, note hair follicles, sebaceous glands], no muscularis mucosae, veins, anal sphincter). | |
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177-3
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Recto anal junction [rectum at far right], H&E, 20X (RECTUM: simple columnar epithelium in glands. ANAL CANAL: initially stratified columnar/cuboidal, then stratified squamous epithelium first non keratinized and then keratinized [thin skin], no muscularis mucosae, veins, anal sphincter). | |
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UCSF 246
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Jejunum, human, H&E stain, 40X | |
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UCSF 247
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Jejunum, human, H&E and silver stain, 40X | |
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207
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Small intestine (Muscularis Externa): Study the orientation of the smooth muscle cells in the intestinal muscularis externa. The micrograph will help you understand the pattern, which arises from the inner circular layer and outer longitudinal layer of smooth muscle cells. Without the knowledge in which direction the intestinal epithelium is located, it is not possible to discriminate between the two sublayers of the muscularis externa. | |
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208
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Small Intestine (Tip of Villus, longitudinal section): The villus is covered by a simple columnar epithelium. Note that cells are sloughing off at the tip of the villus. Find some goblet cells, which represent one resident cell type of the intestinal epithelium. The small lymphocyte is transient and is not a permanent component of the epithelium. Find the “striated border” and realize that you can’t resolve individual microvilli at this magnification. Study the composition of the connective tissue core of the villus. | |
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211
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Small intestine (Base of a Villus in the Jejunum): This micrograph depicts the typical appearance of the epithelium at the base of an intestinal villus. Note the striated or brush border lining at the apical aspect of the simple columnar epithelium and the presence of mucous-secreting cells. | |
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213
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Microvilli: This micrograph shows the typical appearance of microvilli on the apical surface of a cell, which contributes to the striated or brush border lining in the in small intestine. | |
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214
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Crypts of Lieberkühn (Jejunum, cross section): In this simple tubular gland, Paneth cells are found near the end, whereas mucous cells and undifferentiated cells take up the major portion of the gland. Review the reasons for the high rate of cell mitosis in the upper part of the intestinal gland. | |
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216
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Large Intestine (Colon): Goblet cells are particularly numerous in the large intestine. If you look closely at the labeled goblet cell, you can see that the apex is packed with mucus-containing secretory vesicles. A single crypt is seen in the section and its lumen doesn’t stay in the plane of section all the way to the base. |
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1
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Liver, H&E, 40X (central vein, portal triad [portal vein, bile duct, hepatic artery], hepatocyte, sinus, endothelium, Kupffer cell, space of Disse, liver lobule). | |
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188
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Pancreas, H&E, 20X (exocrine pancreas, lobule, acini, centroacinar cell, intercalated duct, endocrine pancreas [islets of Langerhans]). | |
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188B
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Pancreas, H&E, 40X | |
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189
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Pancreas, vascular injection, 40X (vascular pattern, high vascularity of islets). | |
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190B
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Pancreas, chrome alum hematoxylin and phloxine stain, differential staining of cells in islets of Langerhans, 40X (in an islet note that alpha cells [glucagon] have reddish cytoplasm, and beta cells [insulin] have pale blue green cytoplasm; delta cells [somatostatin] can't be distinguished). | |
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194
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Liver and gall bladder, H&E, 40X (LIVER: central vein, portal triad [portal vein, bile duct, hepatic artery], hepatocyte, sinus, endothelium, Kupffer cell, space of Disse, liver lobule. GALL BLADDER: epithelium, lamina propria, muscularis). | |
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195
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Liver and gall bladder, Masson trichrome, 40X (LIVER: distribution of collagen. GALL BLADDER: epithelium, lamina propria, muscularis ). |